- For reproducible and unbiased cDNA synthesis from 5' and 3' ends of mRNAs
- Contains an ultra-stable reverse transcriptase which can synthesize cDNAs up to 9kb
- High cDNA yields from as little as 5pg RNA - easily work with low copy templates
- Broad starting template range - use between 5pg - 5g
- The kit contains a buffer which has pre-optimised levels of oligo dT and random hexamers which results in reduced transcript bias for downstream qPCR
- Short protocol (30mins only)
Convenient two tube format:
- Tube 1: reverse transcriptase with RNase inhibitor to prevent degradation of RNA by contaminating RNases
- Tube 2: buffer concentrate containing oligo dT, random hexamers,MgCl2, dNTPs, enhancers and stabilisers