Purospher™ STAR NH2 (Amino) columns are widely used for carbohydrate analysis, with a typical mobile phase consisting of acetonitrile and water.

In terms of their polarity, these columns lie between bare silica (normalphase chromatography) and reversed-phase silica (reversed-phase chromatography). Hence, Purospher™ STAR NH2 can also be used as an ion-exchanger. In acidic solutions, the NH2-groups are protonated (-NH3+X-) and therefore display the characteristics of a weak anion exchanger.

Medium polarity Purospher™ STAR NH2 columns possess hydrophilic as well as hydrophobic properties, thus can be used under both reversed phase and normal-phase conditions. However, retention is weaker than on silica or RP-supports.

NOTE - END FITTINGS:

With LiChroCART® cartridges the user works with re-usable endfittings which fit different cartridge lengths. Since these cartridge holders may remain in the system and the capillary connections do not need to be detached, the cartridges may be changed within the shortest possible time. Changing a separation cartridge from a length of 125 to 250 mm and back presents no problems. Furthermore, the adaptation of internal diameter (3 mm or 2 mm) to the analysis problem is possible within a few minutes. The endfittings are designed to allow the cartridges to be hand-sealed at normal working pressures of 150 to 200 bar without the need for any tools. Only at higher pressures may further tightening with a wrench become necessary.