TMSH 0.2 M in Methanol - Alkylation (Methylation) Reagent
M: 94.06 g/mol
Methylation with TMSH is recommended for free acids, chlorophenoxycarboxylic
acids, their salts and derivatives as well as for phenols
and chlorophenols. Lipids or triglycerides can be converted
to the corresponding fatty acid methyl esters (FAMEs) by a simple
transesterification.
This reaction is very elegant and convenient, because it is just necessary
to add the reagent (0.2 M in methanol) to the sample solution.
Removal of excess reagent is not required, since in the injector of the
gas chromatograph, at 250°C, pyrolysis to volatile methanol and dimethylsulfide
will occur. Due to the high reactivity, complete derivatization
is often obtained at ambient temperature. However, heating
(e.g., 10 min at 100°C) in a closed sample vial may be necessary to
complete the reaction.