Animal Dope Testing - Racehorse and Greyhound 'Dope' Testing
In animal dope testing, as with human dope testing, the analyst must be aware of how a particular drug is metabolised. A drug will not usually be excreted in an unchanged or parent form and will have probably undergone some chemical modification such as oxidation or the addition of further chemical groups to the basic structure. These groups must often be removed in order to isolate the drug and can be removed either enzymatically or chemically.
Testosterone, for example, is primarily excreted from the horse as the glucuronide (i) and
sulphate (ii) conjugates (Figure 2). Both these side chains must be cleaved in order to isolate testosterone which is then derivatised for detection by GCMS. Derivatisation to the Mox TMS (methoxime-trimethylsilyl) derivative of testosterone gives rise to a chromatogram with sharp peaks from which can be obtained a distinctive mass spectrum.
See the full article at:
http://nzic.org.nz/ChemProcesses/biotech/12B.pdf
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