General Description
Chromolith® Diol columns are more versatile than bare silica columns, and often offer improved reproducibility. The
bonded phase’s hydroxyl groups provide good selectivity without excessive retention. This is due to weaker hydrogen
bonding with diol groups than with silanols on a bare silica surface. In aqueous phases, the diol phase can effectively
shield the silica surface from interacting with proteins.
Diol columns are commonly used for the separation of steroids
and sterols under normal-phase conditions. Chromolith® Diol columns are suitable for the separation of alcohols,
amino acids, carotinoids, oils, glycols, preservatives, proteins, sugars, sulfonamides, and water-soluble vitamins.
Chromolith® is a registered trademark of Merck KGaA, Darmstadt, Germany
