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PCB Analytical Method Solutions | Wellington Laboratories | Greyhound Chromatography

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PCB Analytical Method Solutions

The complex nature of commercial polychlorinated biphenyl (PCB) mixtures is well documented.

The practice of analyzing for marker PCBs (congeners that are most prevalent in technical mixtures) and WHO PCBs (congeners that exhibit “dioxin-like” toxicological activity) has become routine practice in analytical laboratories.  Congener specific analyses are also very important since commercial PCBs can vary in composition on a lot to lot basis and can change once they age in the environment.

Wellington offers method sets, which include calibration kits and support solutions, for the GCMS analysis of PCBs by the following methods:

  • U.S. EPA Method 1668C
  • U.S. EPA Method 1668
  • Environment Canada Method 1/RM/31
  • BS-EN 1948-4:2010 (previous draft method = prCEN/TS 1948-4)

The electrophilic chlorination of biphenyl leads to complex mixtures of chlorobiphenyl (PCB) congeners ranging from monochloro- up to decachloro-biphenyl. Although 209 PCB congeners are theoretically possible, only about 150 are found in industrial mixtures and have ended up in the environment.

Certain PCB congeners are considered more toxic than others and are termed ‘dioxin-like’. For this reason there are analytical methods that target only these ‘dioxin-like’ PCBs, while other methods focus on the major PCB congeners in the industrial mixtures. In addition, there are methods that address both groups of PCB congeners.

Wellington has designed, prepared, and offers a number of sets of calibration kits and support solutions for the analysis of individual PCB congeners. 


This set of solutions is to be used for the analysis of the 12 dioxin-like PCB congeners by HRGC/HRMS.

EPA Method 1668C

This series of calibration solutions, and corresponding support solutions, were prepared to be used according to U.S. EPA Method 1668, Revision C.

EPA Method 1668

This calibration kit and support solutions were designed and prepared to be used with the Draft version (March, 1997) of U.S. EPA Method 1668 which is still popular with some laboratories.


Environment Canada Method 1/RM/31 is a HRGC/LRMS method for PCB analysis and these solutions were prepared to be used with this method.

P48-W-CVS and P48-M-CVS

European Standard Method EN 1948-4 is to be used for analysis of the 12 ‘dioxin-like’ PCB congeners and the 6 ‘marker’ PCB congeners in stationary source emissions. These two calibration kits, and their support solutions, were prepared for this method.


This calibration set is a combination of P48-W-CVS and P48-M-CVS.


We also offer solutions to test the resolution of common HRGC columns for 2,3,7,8-TCDD and 2,3,7,8-TCDF and set retention time windows for PCDD and PCDF congener groups.

Wellington Laboratories Catalogue Image

Full range of PCB Analytical Method Solutions Reference Standards can be found on Pages 65 - 78 of the current catalogue. 

Browse the full catalogue for:


  • PCDD/PCDF Analytical Method Solutions
  • Individual PCDDs & PCDFs: Native and Mass-Labelled
  • Specialty Solution/Mixtures of PCDDs and PCDFs
  • PCB Analytical Method Solutions
  • Individual PCBs: Mass-Labelled
  • Specialty Solution/Mixtures of PCBs
  • Halogenated Flame Retardants & Related Compounds
  • Perfluorinated Compounds (PFCs),
  • Certified Reference Materials

Additional Products


  • PAH Calibration Sets and Native Mass-Labelled Support Solutions
  • Chlorinated Biphenylols (HO-PCBs), Native and Mass-Labelled Individuals, Mass Labelled Solutions & Mixtures
  • Mass-Labelled Chlorinated Biphenylols Solutions/Mixtures
  • Methoxy Chlorobiphenyls (MeO-PCBs), Native and Mass-Labelled Individuals and Solution/Mixtures
  • Triclocarban, Native and Mass-Labelled
  • Triclosan, Methyl Triclosan, Native and Mass-Labelled and Chlorinated Derivatives
  • Tris(4-chlorophenyl) Methane and Methanol
  • Chlorinated Naphthalenes (PCNs), Native Individuals and Solutions/Mixtures
  • Chlorinated Diphenyl Ethers (PCDEs), Native and Mass-Labelled
  • Native and Mass-Labelled Chlorobenzene and Chlorophenol Solution/Mixtures
  • Mass-Labelled Chlorobenzenes, Chlorophenols, Chlorocatechols, and Chloroveratroles
  • Native Melamine & Cyannuric Acid
  • Native and Mass-Labelled Bishenol A and Native Bisphenol Analogues
  • Native and Mass-Labelled Tetrachlorodibenzothiophenes
  • Native and Mass Labelled Halogenated Carbazoles


Guidelines for the use and Handling of Wellington products

General Structure and Numbering System of Selected Aromatic Hydrocarbons

Number of Possible Isomers for Selcted Halogenated Aromatic Compounds

Exact Mass & Ralative Ion Abundance of Selected Chlorinated Aromatic Hydrocarbons

Molecular Ion Clusters for Cholorinated Aromatic Hydrocarbons

Exact Mass & relative Ion Abundance of Selected Brominated Aromatic Hydrocarbons

Systematic Numbering of Chlorinated Dibenzo-p-Dioxins

Systematic Numbering of Chlorinated Dibenzofurans

Systematic Numbering of Chlorinated  Biphenyls

Systematic Numbering of Chlorinated Naphthalenes


Wellington Laboratories Quality Management


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The people at Wellington are committed to the distribution of quality products as well as the maintenance of excellent customer service. In fact, in order to provide you with the best possible service, we now have three ISO certifications (ISO 9001:2015, ISO/IEC 17025:2017 and ISO 17034:2016) which cover all aspects of planning, production, testing, distribution, and post-distribution service. 

These certifications allow us to monitor and maintain the highest level of quality and service and also allow our customers to satisfy the requirements of their own ISO certifications. 

Our ISO/IEC 17025:2017 accreditation has been certified by the Canadian Association for Laboratory Accreditation Inc. (CALA) and our scope is available for review on the CALA Directory of Accredited Laboratories (http://www.cala.ca). 

Similarly, our ISO 17034:2016 accreditation has been certified by ANSI National Accreditation Board (ANAB) and our certificate and scope are also available on their website (http://anab.org/). 

If you require a hard copy of any of our ISO certificates, please do not hesitate to contact us at info@well-labs.com

Brock G. Chittim Colleen H.M. Tashiro Nicole Riddell


Wellington Laboratories ISO Certificate   Wellington Laboratories Guide 34 Certificate  

 Wellington Laboratories GC MS Reference Handling Guide    Wellington Laboratories PFC Reference Handling Guide   Wellington Laboratories PFC quick Reference Guide 

PFAS EBook Image PFAS Environmental EBook


Tel:        +44 (0) 151 649 4000  

Web:     www.greyhoundchrom.com  

Email:   marketing@greyhoundchrom.com  


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